Overproduction of Laccase and Pectinase by Microbial Associations in Solid Substrate Fermentation |
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Authors: | Ivanka Stoilova Albert Krastanov |
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Institution: | (1) Department of Biotechnology, University of Food Technologies, 4002 Plovdiv, Bulgaria |
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Abstract: | The growth and the enzymatic production of two microbial fungal associations were studied: Aspergillus niger and Fusarium moniliforme and Trametes versicolor and Aspergillus niger. The synergistic interrelations between the species of the first mixed culture increased the biosynthesis of α-amylase and
pectinase. T. versicolor and A. niger proved to be compatible partners in the overproduction of the enzyme laccase, whose synthesis surpassed 8.4 times the enzymatic
level in the monoculture, with both of the mixed microbial populations cocultivation facilitating the amplified synthesis
of enzymes rather than their growth acceleration. A further proof of the presence of synergism established by the cultures
was the enzyme volumetric productivities in both of the mixed microbial cultures, which increased parallel to the rise in
the combined biomass synthesis. The competent selection of compatible partners can adjust the desired enzymatic levels and
compositions in mixed fungal systems aimed at a number of specified designations. Thus, a very high level of laccase production
(97,600 IU/g dry weight) was achieved. The chosen fungal strains produce a variety of different enzymes, but first microbial
association produces mainly amylase and pectinase, necessary for their growth, and second association produces mainly laccase
and pectinase. |
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Keywords: | Solid substrate fermentation Laccase Pectinase Mixed fungal culture |
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