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| 间接法测定美斯替仑磷脂复合物的复合率 | |
| 引用本文： | 陈学梁,谭群友,尹华峰,朱照静,张景勍.间接法测定美斯替仑磷脂复合物的复合率[J].光谱实验室,2012,29(1):116-119. |
| 作者姓名： | 陈学梁 谭群友 尹华峰 朱照静 张景勍 |
| 作者单位： | 1. 重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室 400016 2. 第三军医大学大坪医院野战外科研究所胸外科 400042 3. 重庆医药高等专科学校 400030 |
| 基金项目： | 教育部博士点基金资助项目，重庆市教育委员会资助项目(首批高等学校优秀人才资助 |
| 摘 要： | 建立美斯替仑磷脂复合物复合率的间接测定方法。采用紫外分光光度法测定未参与复合的美斯替仑的量,由此计算复合率。结果表明,美斯替仑在269nm处有最大吸收,浓度在10.02—40.09μg·mL-1时与吸光度呈良好的线性关系,回归方程为:A=9.0176C+0.0065,r=0.9999（n=3）;平均回收率为99.54%,RSD为0.66%。本法操作简便易行,具有良好的重现性和精密度。 |
| 关 键 词： | 美斯替仑 磷脂复合物 间接法 复合率 |
Determination of Complexation Rate of Mestinon Phospholipid Complex by Indirect Method |
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| CHEN Xue-Liang , TAN Qun-You , YIN Hua-Feng , ZHU Zhao-Jing , ZHANG Jing-Qing.Determination of Complexation Rate of Mestinon Phospholipid Complex by Indirect Method[J].Chinese Journal of Spectroscopy Laboratory,2012,29(1):116-119. | |
| Authors: | CHEN Xue-Liang TAN Qun-You YIN Hua-Feng ZHU Zhao-Jing ZHANG Jing-Qing |
| Institution: | Qing (Medicine Engineering Research Center,Chongqing Key Laboratory of Biochemical & Molecular Pharmacology, Chongqing Medical University,Chongqing 400016,P.R.China) a(Center of Thoracic Surgery,Duping Hospital & Research Institute of Surgery,Third Military Medical University, Chongqing 400042,P.R.China) b(Chongqing Medical And Pharmaceutical College,Chongqing 400030,P.R.China) |
| Abstract: | The indirect method for determination of the complexation rate of mestinon phospholipid complex was established.The content of the not compound mestinon was determined by UV spectrophotometry,basing on which the complexation rate was calculated.The result showed that the mestinon had a maximum absorption at 269nm.The mestinon had a good linear relationship with absorbance in the range of 10.02—40.09μg · mL-1.The regression equation was as follows: A = 0.0176C+ 0.0065,r= 0.9999（n= 3）.The average recovery was 99.54%with RSD of 0.66%. The method is simple with good accuracy and precision. |
| Keywords: | Mestinon Phospholipid Complex Indirect Method Complexation Rate |
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