| 毛泡桐花中总黄酮含量的测定 |
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| 引用本文： | 孟志芬,祝勇,李聪,李亚娟.毛泡桐花中总黄酮含量的测定[J].光谱实验室,2012,29(1):221-225. |
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| 作者姓名： | 孟志芬 祝勇 李聪 李亚娟 |
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| 作者单位： | 河南科技学院化学化工学院 453003 |
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| 基金项目： | 河南省高等学校青年骨干教师资助计划资助项目 |
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| 摘 要： | 以70%乙醇为溶剂回流提取了毛泡桐花中黄酮类化合物,用3种显色方法对其紫外可见光谱进行了定性研究。结果表明,在硝酸铝显色法中,芦丁标准品和样品溶液的吸收峰一致,并且该方法稳定性好,精密度高(RSD=1.37%),重现性好(RSD=5.57%),回收率高(RSD=3.74%),操作简单方便,可适用于毛泡桐花提取液中总黄酮含量的测定。
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| 关 键 词： | 毛泡桐花 总黄酮 紫外-可见分光光度法 |
Determination of Total Flavonoids from Paulownia tomentose steud Flower |
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| MENG Zhi-Fen
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ZHU Yong
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LI Cong
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LI Ya-Juan.Determination of Total Flavonoids from Paulownia tomentose steud Flower[J].Chinese Journal of Spectroscopy Laboratory,2012,29(1):221-225. |
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| Authors: | MENG Zhi-Fen ZHU Yong LI Cong LI Ya-Juan |
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| Institution: | (Department of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang, Henan 453003,P.R.China) |
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| Abstract: | The total flavonoids from Paulownia tomentnose steud flower were extracted by 70% alcohol.The ultraviolet-visible spectra were studied by three colorimetry.The results showed that in aluminum nitrate colorimetry,the absorption peaks of rutin standard solution and sample were the same.The method had high stability,precision(RSD = 1.37%),replicability(RSD = 5.57%), recovery(RSD= 3.74%) with simple operation.The method was applicable to determination of the content of total flavonoids from paulownia tomentnose steud flower. |
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| Keywords: | Paulownia tomentose steud Flower Total Flavonoids Ultraviolet-Visible Spectrophotometry |
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