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Electrochemical Estrogen Receptor α based Biosensor for Label‐Free Detection of Estradiol |
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| Authors: | Zhenzhong Guo Nadia Zine Patrick Balaguer Aidong Zhang Philippe Namour Florence Lagarde Nicole Jaffrezic‐Renault |
| Institution: | 1. University of Lyon, Institute of Analytical Sciences, UMR‐CNRS 5280, 5, Rue de La Doua, 69100 Villeurbanne, France phone: +33437423558;2. IRCM, Institut de Recherche en Cancérologie de Montpellier, Montpellier, F‐34298, France;3. Key Laboratory of Pesticides and Chemical Biology, Huazhong Normal University, Luoyu Str 152, Hongshan District, 430079 Wuhan, P.?R. China |
| Abstract: | We describe a new electrochemical biosensor based on estrogen receptor α (ER‐α) for label‐free detection of 17β‐estradiol, a model of endocrine‐disrupting compounds. ER‐α is coupled onto the gold electrode through its 6‐His tag and NTA‐copper complex. After interaction of estradiol with ER‐α, the biosensor presents a well‐defined peak at +500 mV due to estradiol oxidation (E17 peak). The linear range of detection is from 1 fM to 1 nM and the detection limit is 1 fM. Good selectivity was obtained for interfering substances at nanomolar level, for concentration of E17 up to 0.1 pM. The E17 was detected in hospital effluents. |
| Keywords: | Estradiol Estrogen receptor α Square‐wave voltammetry NTA‐copper complex |