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A Ready‐to‐Use Electrochemical Kit Design for the Diagnosis of Single Nucleotide Polymorphisms |
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| Authors: | Yasin Ugur Kayran Dilsat Ozkan‐Ariksoysal Burcin Tezcanli Buket Kosova Mehmet Ozsoz |
| Affiliation: | 1. Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey tel: +90?232?311?13?53, fax: +90?232?388?52?58;2. Department of Medicinal Biology, Faculty of Medicine, Ege University, 35100, Bornova, Izmir, Turkey;3. Department of Electric and Electronic Engineering, Nanotechnology Center, Gediz University, 35665, Seyrek, Izmir, Turkey. |
| Abstract: | In this study, for the first time a model electrochemical kit was constructed for the detection of a functional polymorphism in catechol‐O‐methyl transferase (COMT) gene which is important for diagnosis of neuropsychiatric disorders as Alzheimer disease. The disposable pencil graphite electrode (PGE) is designed as a “kit” and the probe DNA covered PGE can detect single nucleotide polymorphisms (SNPs) from real samples based on the guanine oxidation signal even after 5 months of kit preparation (150 days durability).The detection limit (S/N=3) of the biosensor was calculated as 1.18 pmol of synthetic target sequence and 6.09×105 molecules of real samples in 30 min detection time. |
| Keywords: | Electrochemical DNA biosensor Point‐of‐care test Ready‐to‐use kit design Single nucleotide polymorphism Catechol‐O‐methyl transferase (COMT) Val108/158 Met polymorphism |