Chitosan/Ionic Liquid Composite Electrode for Electrochemical Monitoring of the Surface‐Confined Interaction Between Mitomycin C and DNA |
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| Authors: | Ece Eksin Mihrican Muti Arzum Erdem |
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| Institution: | 1. Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100, Izmir, Turkey phone: +90‐232‐311?5131, fax: +90‐232‐388?5258;2. Adnan Menderes University, Faculty of Science, Chemistry Dept., 09010 Ayd?n, Turkey |
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| Abstract: | In the present study a chitosan/ionic liquid modified pencil graphite electrode (CHIT‐IL‐PGEs) was developed for the first time for enhanced electrochemical monitoring of nucleic acid, and the interaction of the anticancer drug Mitomycin C (MC) and calf thymus double stranded DNA (dsDNA) by measuring the oxidation signals of MC and guanine in the same voltammetric scale. Differential pulse voltammetry, cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to evaluate the performance of the CHIT‐IL based biosensor on electrochemical monitoring of DNA, and drug‐DNA interaction. The experimental parameters, IL, dsDNA and MC concentration and the interaction time were then optimized. |
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| Keywords: | Chitosan drug‐DNA interaction Ionic liquid Mitomycin C Electrochemical sensors |
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