Effective and Functional Surface Design for Biosensing Applications Based on a Novel Conducting Polymer and PMMA/Clay Nanocomposite |
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| Authors: | Melis Kesik Ozgecan Kocer Fulya Ekiz Kanik Naime Akbasoglu Unlu Eda Rende Evren Aslan‐Gurel Rene M. Rossi Yasemin Arslan Udum Levent Toppare |
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| Affiliation: | 1. Department of Chemistry, Middle East Technical University, 06800 Ankara, Turkey;2. Department of Biotechnology, Middle East Technical University, 06800 Ankara, Turkey;3. EMPA Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Protection and Physiology, 9014 Lerchenfeldstrasse 5, St.Gallen, Switzerland;4. Institute of Science and Technology, Department of Advanced Technologies, Gazi University, 06570 Ankara, Turkey;5. Department of Polymer Science and Technology, Middle East Technical University, 06800 Ankara, Turkey;6. The Center for Solar Energy Research and Application (GüNAM), Middle East Technical University, 06800 Ankara, Turkey |
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| Abstract: | Surface functionalization plays a crucial role in the design of biosensors. For this purpose, a novel functional monomer, 6‐(4,7‐bis(2,3‐dihydrothieno[3,4‐b][1,4]dioxin‐5‐yl)‐2H‐benzo[d][1,2,3]triazol‐2‐yl)hexan‐1‐amine (BEDOA‐6), was designed and synthesized. Poly(BEDOA‐6) was utilized as an immobilization matrix for glucose oxidase biosensor construction. Moreover, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in situ suspension polymerization. Conducting polymer surface was modified with PMMA/clay nanocomposite material and a glucose biosensor was developed. In addition, XPS and SEM were utilized to characterize the surface properties. The biosensor shows a wide linear range between 2.8 µM and 1.2 mM to glucose with a low detection limit of 1.99 µM. Finally, the biosensor was tested on serum samples containing actual human blood. The results were in well‐agreement with a reference method. |
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| Keywords: | Biosensors Conducting polymers PMMA Nanocomposites Laponite |
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