Interaction Between DNA and Some Salicylic Acid Derivatives and Characterization of Their DNA Targets |
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| Authors: | M. Abedi S. Z. Bathaie M. F. Mousavi |
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| Affiliation: | 1. Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran;2. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | A nanofiber polypyrrole (PPy) film was electrochemically deposited on a Pt electrode and used for immobilization of single‐stranded DNA (ssDNA) and investigation of hybridization events. Then, the interaction of DNA with four salicylic acid (SA) derivatives was studied with electrochemical methods. The oxidation peak of guanine was decreased by increasing the concentrations of salicylic acid derivatives. The binding constants of these compounds with four different sequences of DNA including different percentages of guanine‐cytosine and adenine‐thymine bases were calculated and it was clarified that sequences with higher percentage of adenine‐thymine bases have a higher binding constant in their interaction with SA derivatives. |
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| Keywords: | Electrochemical biosensors Nucleic acids Drugs Nanoparticles Polymers |
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