Clerodendrumic Acid,a New Triterpenoid from Clerodendrum glabrum (Verbenaceae), and Antimicrobial Activities of Fractions and Constituents |
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| Authors: | Ndivhaleni A Masevhe Maurice D Awouafack Aroke S Ahmed Lyndy J McGaw Jacobus N Eloff |
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| Institution: | 1. Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa, (phone/fax: +27?(0)?12 529 8525);2. Department of Botany, School of Mathematics and Natural Sciences, University of Venda, Thohoyandou 0950, South Africa;3. Laboratory of Natural Products Chemistry, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon |
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| Abstract: | One new triterpenoid, (3β,11α,19β)‐3‐(butanoyloxy)‐11‐hydroxytaraxast‐20(30)‐ene‐23,28‐dioic acid (clerodendrumic acid; 1 ) was isolated from the hexane extract of the leaves of Clerodendrum glabrum var. glabrum along with heptadecanoic acid ( 2 ). The structure of the new compound was elucidated by interpretation of its NMR (1D and 2D), MS, and IR data. Combined fractions C and D from the column chromatography of the hexane extract exhibited significant antifungal activities (average MIC of 0.10 mg/ml) against Candida albicans and Cryptococcus neoformans. C. albicans was relatively resistant to clerodendrumic acid ( 1 ; MIC 125 μg/ml) and was resistant to heptadecanoic acid ( 2 ; MIC 188 μg/ml). Both compounds had low antibacterial activities against two Gram‐positive and two Gram‐negative bacteria with average MIC values of 157 and 172 μg/ml, respectively. Compounds 1 and 2 were relatively nontoxic against monkey kidney Vero cells in vitro with IC50 values of 202.6 and 108.4 μg/ml, respectively. |
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| Keywords: | Clerodendrum glabrum Clerodendrumic acid Antimicrobial activity Cytotoxic activity |
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