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Preventing the mixed detergent micelle effect in two‐dimensional electrophoresis on Tris‐Tricine gels |
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| Authors: | Hana Kone?ná Dana Fridrichová Gabriela Lochmanová Jan Bárta Zbyněk Zdráhal |
| Institution: | 1. Central European Institute of Technology, Masaryk University, , Brno, Czech Republic;2. NCBR, Masaryk University, , Brno, Czech Republic;3. Department of Plant Production and Agroecology, Faculty of Agriculture, University of South Bohemia, , ?eské Budějovice, Czech Republic |
| Abstract: | Application of Tris‐N‐Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS–SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation. |
| Keywords: | 2DE Mixed detergent micelles Tris‐Tricine gel |