Fluorescent staining of protein in SDS polyacrylamide gels by salicylaldehyde azine |
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| Authors: | Guo‐Ying Hong Zhong‐Xin Zhu Yuan‐Meng Duan Xuan Zhou Li‐Tai Jin |
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| Affiliation: | 1. Zhejiang Provincial Key Laboratory of Biopharmaceuticals, Wenzhou Medical College, , Wenzhou, Zhejiang, P. R. China;2. Wenzhou Undersun Biotechnology Co., Ltd, , Wenzhou, Zhejiang, P. R. China;3. Agricultural College, Anhui Agricultural University, , Hefei, Anhui, P. R. China;4. School of Basic Medical Sciences, Jilin University, , Changchun, Jilin, P. R. China |
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| Abstract: | As a noncovalent fluorescence probe, in this study, salicylaldehyde azine (SA) was introduced as a sensitive fluorescence‐based dye for detecting proteins both in 1D and 2D polyacrylamide electrophoresis gels. Down to 0.2 ng of single protein band could be detected within 1 h, which is similar to that of glutaraldehyde‐silver stain, but approximately four times higher than that of SYPRO Ruby fluorescent stain. Furthermore, comparative analysis of the MS compatibility of SA stain with SYPRO Ruby stain indicated that SA stain is compatible with the downstream of protein identification by LC‐MS/MS. Additionally, the probable mechanism of the SA stain was investigated by molecular docking. The results demonstrated that the interaction between SA and protein was mainly contributed by hydrogen bonding and hydrophobic forces. |
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| Keywords: | Fluorescent stain Polyacrylamide gel electrophoresis Protein detection Salicylaldehyde azine |
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