Fingerprints of Phalaenopsis Tissues in Growth and Spike Induction Periods—A Solid‐state 13C NMR Approach |
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Authors: | Der‐Lii M. Tzou Lin‐Kai Ni Mei‐Man Chen Min‐Chuan Chiou Li‐Chou Chen Shan‐Te Hsu Kuo‐Lung Ku Chien‐Chung Cheng |
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Affiliation: | 1. Department Institute of Chemistry, Academia Sinica, Taipei 11529, Taiwan, ROC;2. Department of Applied Chemistry, National Chia‐Yi University, Chia‐Yi 60004, Taiwan, ROC;3. Department of Horticulture, National Chia‐Yi University, Chia‐Yi 60004, Taiwan, ROC |
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Abstract: | Solid‐state Nuclear Magnetic Resonance (ss‐NMR) 13C single‐pulse excitation spectroscopy in combination with the magic‐angle spinning (MAS) technique was applied to a series of Phalaenopsis tissues, including the leaf, sheath, stem, and root, at different growth and spiking periods. Compared with{1H}/13C cross‐polarization MAS spectra, the 13C single‐pulse excitation MAS spectra displayed very distinct spectral patterns, recognizable as fingerprints of the tissues studied. 1Here, we demonstrate that solid‐state 13C single‐pulse excitation NMR spectroscopy provides a direct and robust analytical tool for studying the various tissues of Phalaenopsis in different growth and spiking induction periods. |
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Keywords: | Solid‐state NMR Phalaenopsis Cross‐polarization Spike Magic‐angle spinning |
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