Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking |
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| Authors: | Yuanqi Lu Hong Bai Chunyan Kong Hao Zhong Michael C. Breadmore |
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| Affiliation: | 1. Analysis and Testing Centre, Dezhou University, , Dezhou, P. R. China;2. Institute of Materia Medica, Shandong Academy of Medical Sciences, , Jinan, P. R. China;3. Key Laboratory of Coordination Chemistry and Functional Materials in Universities of Shandong, Dezhou University, , Dezhou, P. R. China;4. Austrialian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, , Hobart, Tasmania, Australia |
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| Abstract: | A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample‐to‐sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum. |
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| Keywords: | Acid barrage stacking (ABS) Brazilin CE Protosappanin B |
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