Detection of Prunus Necrotic Ringspot Virus in Plant Extracts with Impedimetric Immunosensor based on Glassy Carbon Electrode |
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| Authors: | Urszula Jarocka Hanna Radecka Tadeusz Malinowski Lech Michalczuk Jerzy Radecki |
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| Institution: | 1. Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10‐748 Olsztyn, Poland;2. Research Institute of Horticulture, Konstytucji 3 Maja 1/3, 96‐100 Skierniewice, Poland |
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| Abstract: | This paper concerns the development of an immunosensor for detection of Prunus necrotic ringspot virus (PNRSV) in plant extracts. The immunosensor fabrication consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) immobilization of anti‐PNRSV IgG polyclonal antibody, (iiii) filling free spaces with BSA. Each step was controlled with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The immunosensor was applied for the determination of the PNRSV virus in extracts from cucumber leaves using EIS technique. It was capable of discriminating between samples consisting of extracts from healthy plants and consisting of leaf extracts from infected plants diluted 10 000 times with extract from healthy plants. |
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| Keywords: | Prunus necrotic ringspot virus Glassy carbon electrodes Immunosensors Electrochemical impedance spectroscopy |
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