G‐Quadruplex‐Linked Supersandwich DNA Structure for Electrochemical Amplified Detection of Thrombin |
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Authors: | Guangfeng Wang Xiuping He Yanhong Zhu Ling Chen Lun Wang Xiaojun Zhang |
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Affiliation: | 1. College of Chemistry and Materials Science, Anhui Key Laboratory of Chem‐Biosensing, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, Wuhu 241000, P.?R. China tel: +86 (0)553‐3883513;2. fax: +86 (0)553‐3869302 |
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Abstract: | In this paper, a novel strategy of electrochemical amplified detection of thrombin based on G‐quadruplex‐linked supersandwich structure was described. In the presence of K+ and hemin, the original hairpin DNA sequence activated an autonomous cross‐opening process to build up hemin/G‐quadruplex structure and can hybridize to form supersandwich structure containing multiple signal labels. With the addition of thrombin, it conjugated with its aptamer, leading to a remarkably descended signal. The supersandwich‐amplified electrochemical sensor system was highly sensitive in the concentration range from 10?6 to 10?10 M with a detection limit of 10 pM and also demonstrated excellent selectivity. The amplifying supersandwich structure with multiple labels can be implemented as a versatile sensing platform for analyzing other DNA in the presence of the appropriate probe. |
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Keywords: | Thrombin G‐quadruplex DNA Supersandwich Aptasensor |
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