Electrochemical Detection of NADH,Cysteine, or Glutathione Using a Caffeic Acid Modified Glassy Carbon Electrode |
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Authors: | P. T. Lee R. G. Compton |
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Affiliation: | Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ, UK |
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Abstract: | A modified electrode was prepared using electrodeposition methods to immobilize caffeic acid (CAF) onto the surface of a glassy carbon electrode (GCE) to create a polymer suitable for biosensor development. The polymer film coverage of the surface bound species was further optimized using electrodeposition methods, thus increasing the surface coverage to ca. 10?9 mol cm?2. Using cyclic voltammetry, the modified carbon electrode was used to facilitate and observe the electrocatalytic oxidation of coenzymes such as NADH, cysteine, and glutathione at different concentrations. A calibration curve was determined in each case within the concentration range; 300 nM to 10 mM, with the limits of detection (LOD) of 246 µM, 99 µM, 2.2 µM for NADH, cysteine, and glutathione respectively. |
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Keywords: | Keywords: Caffeic acid Modified carbon electrode Surface immobilization Electrocatalytic oxidation Cyclic voltammetry |
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