Amperometric Detection of Histamine with a Pyrroloquinoline‐Quinone Modified Electrode |
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Authors: | Joshua A. Young Xiayu Jiang Jon R. Kirchhoff |
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Affiliation: | Department of Chemistry, College of Natural Sciences and Mathematics, University of Toledo, 2801 W. Bancroft Street, Toledo, OH 43606, USA |
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Abstract: | A modified glassy carbon (GC) electrode was developed for the amperometric detection of biogenic amines, particularly histamine. The electrode was modified with the co‐enzyme pyrroloquinoline quinone (PQQ) by entrapment during electropolymerziation of pyrrole to form polypyrrole (PPy). This method formed a thin film on the electrode surface possessing very good stability with a shelf‐life exceeding one month without loss of signal. Optimal conditions for the PQQ/PPy electrode were determined and a linear response was found for histamine in phosphate buffer (pH 6) at +550 mV from 40 to 170 mg L?1 with a limit of detection (S/N≥3) of 38 mg L?1. The practical linear range offered by this method suggests ideal use for spoilage detection in fermented foods. |
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Keywords: | Histamine Amperometric biosensors Quinone electrocatalysts Polypyrrole |
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