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Electrochemical Detection of Avian Influenza Virus Genotype Using Amino‐ssDNA Probe Modified Gold Electrodes |
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| Authors: | Kamila Malecka Iwona Grabowska Jerzy Radecki Anna Stachyra Anna Góra‐Sochacka Agnieszka Sirko Hanna Radecka |
| Institution: | 1. Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10‐748 Olsztyn, Poland;2. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A, 02‐106 Warsaw, Poland |
| Abstract: | A DNA biosensor for the detection of specific oligonucleotide sequences of Avian Influenza Virus type H5N1 has been proposed. The NH2‐ssDNA probe was deposited onto a gold electrode surface to form an amide bond between the carboxyl group of thioacid and the amino group from ssDNA probe. The signals generated as a result of hybridization were registered in square wave voltammetry and electrochemical impedance spectroscopy in the presence of Fe(CN)6]3?/4? as a redox marker. The genosensor is capable to determine 20‐mer and 180‐bp (PCR products) oligonucleotides complementary sequences with detection limit in the fM range. The genosensor displays good selectivity and sensitivity. The 20‐mer as well as 180‐bp oligonucleotides without a complementary sequence generate very low signal. |
| Keywords: | Electrochemical genosensor Amino‐ssDNA probe PCR products Avian Influenza Virus Gold electrode |