Label‐free aptamer‐based partial filling technique for enantioseparation and determination of dl‐tryptophan with micellar electrokinetic chromatography |
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| Authors: | Rong Huang Wenming Xiong Daifang Wang Longhua Guo Zhenyu Lin Lishuang Yu Kedan Chu Bin Qiu Guonan Chen |
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| Institution: | 1. Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety and Department of Chemistry, Fuzhou University, , Fuzhou, Fujian, China;2. College of Pharmacy, Fujian University of Traditional Chinese Medicine, , Fuzhou, Fujian, China |
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| Abstract: | In this study, a simple and reproducible method for enantioseparation and determination of dl ‐tryptophan (dl ‐T rp) was developed by using a partial filling technique in combination with MEKC . The corresponding l ‐T rp specific DNA aptamer was used as a chiral selector. Sodium cholate was used to form the chiral micelles and to enhance the enantioseparation of the enantiomers. Effects of aptamer concentration, filling time, buffer composition, and separation voltage on the enantioseparation were evaluated. The M g2+ and Na+ concentration in separation buffer was found to effectively affect the separation efficiency and reproducibility. Under the optimal conditions, d ‐ and l ‐T rp were completely enantioseparated in less than 9 min. This aptamer‐based partial‐filling approach has the potential to be extended to the separation of other enantiomers after the replacement of corresponding specific aptamers. |
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| Keywords: | DNA aptamer Enantioseparation MEKC Partial filling technique dl‐Tryptophan |
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