Hemin Functionalized Multiwalled Carbon Nanotubes as a Matrix for Sensitive Electrogenerated Chemiluminescence Cholesterol Biosensor |
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Authors: | Xiaoping Wu Yaqin Chai Ruo Yuan Shihong Chen Meihe Zhang Juanjuan Zhang |
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Affiliation: | Education Ministry Key Laboratory on Luminescence and Real‐Time Analysis, Key Lab of Chongqing Modern Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P.?R. China |
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Abstract: | Based on hemin‐MWCNTs nanocomposite and hemin‐catalyzed luminol‐H2O2 reaction, a sensitive electrogenerated chemiluminescence (ECL) cholesterol biosensor was proposed in this paper. Firstly, hemin‐MWCNTs was prepared via π–π stacking and modified on the surface of GCE. Subsequently, cholesterol oxidase (ChOx) was adsorbed on the modified electrode to achieve a cholesterol biosensor. Hemin‐MWCNTs nanocomposite provided the electrode with a large surface area to load ChOx, and endowed the nanostructured interface on the electrode surface to enhance the performance of biosensor. The biosensor responded to cholesterol in the linear range from 0.3 µM to 1.2 mM with a detection limit of 0.1 µM (S/N=3). |
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Keywords: | Cholesterol biosensors Nanocomposites Multiwalled carbon nanotubes Hemin‐catalyzed luminol‐hydrogen peroxide reaction Electrogenerated chemiluminescence |
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