Isolation and purification of lignoperoxidase from the mushroom <Emphasis Type="Italic">Pleurotus ostreatus</Emphasis> |
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| Authors: | Sh Ya Mirzaakhmedov Zh F Ziyavitdinov Z R Akhmedova A B Saliev D T Ruzmetova S T Azizov Dimitrios Fessas Stefania Iametti |
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| Institution: | (1) Institute of Bioorganic Chemistry, Uzbekistan, Tashkent;(2) Institute of Microbiology, Uzbekistan, 700128 Tashkent, ul. A. Kadyri, 7b;(3) Institute of Electronics, Uzbekistan, 100125 Tashkent, ul. F. Khodzhaeva, 33;(4) University of Milan, DISTAM, via Celoria 2, 20133 Milano, Italy |
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| Abstract: | The accumulation dynamics of lignolytic enzymes in culture media of the basidiomycetes Panus tigrinus, Pleurotus ostreatus, Fomes fomentarus, and the micromycete Aspergillus terreus were studied during the incubation period. It was found that Pleurotus ostreatus is the most active producer of lignoperoxidase enzymes among the studied fungi. Gel filtration and ion-exchange chromatography
were used to isolate a homogeneous enzyme with lignoperoxidase activity. The maximum activity was found at pH 2.7 and 29°C.
Gel electrophoresis determined the molecular weight (44 kDa).
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Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 564–566, November–December, 2007. |
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| Keywords: | ligninase Pleurotus ostreatus lignoperoxidase activity chromatography electrophoresis |
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