Counter-ionic effect on the separation of water-soluble compounds applying a hydrophilic stationary phase bonded with a zwitter-ionic polymer |
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Authors: | Kamichatani Waka Inoue Yoshinori Saito Mitsuru Kobayashi Yasuyuki Yamamoto Atsushi |
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Institution: | Nippon Filcon Co., Ltd., 2220 Ohmaru, Inagi, Tokyo 208-8577, Japan. w-kamichatani@filcon.co.jp |
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Abstract: | A novel hydrophilic stationary phase bonded with a zwitter-ionic polymer for HPLC was synthesized. The stationary phase, in combination with a mobile phase containing various salts, was evaluated for its ability to separate water-soluble compounds, such as nucleobases, nucleosides and glycosides. The retention of a large majority of the solutes, except for cytosine, was increased by adding anti-chaotropic ions to the mobile phase. These results suggested that the retention of solutes depended on the thickness of the hydration layer on the stationary phase. In the zwitter-ionic polymer adsorbent, the formation of the hydration layer and the ionicity of the zwitter-ionic group on the stationary phase will be controlled by the properties of the ions added to the mobile phase. |
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