| HPLC与UPLC色谱条件转换方法研究 |
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| 引用本文： | 周新,陈会明,白桦,于文莲,王超,郑健,张青,陈伟,马强.HPLC与UPLC色谱条件转换方法研究[J].分析试验室,2008,27(4):56-58. |
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| 作者姓名： | 周新 陈会明 白桦 于文莲 王超 郑健 张青 陈伟 马强 |
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| 作者单位： | 1. 中国检验检疫科学研究院工业品检验研究所,北京,100025
2. 北京市科学技术研究院,北京市理化分析测试中心,北京,100089 |
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| 摘 要： | 以分离分析化妆品中对羟基苯甲酸酯类防腐剂为内容,对HPLC和UPLC的色谱条件转换方法进行了研究。比较了采取不同方法转换得到的色谱条件参数的分离状况,选出了UPLC法最佳色谱条件。同时,用HPLC法和UPLC法对4种化妆品中的4种对羟基苯甲酸酯类防腐剂进行了定量测定,两种方法的测定结果吻合较好,相互印证了方法的准确性。
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| 关 键 词： | 超高效液相色谱 色谱条件转换 化妆品 防腐剂 |
| 文章编号： | 1000-0720(2008)04-056-03 |
| 修稿时间： | 2006年10月28 |
Study on conversion of chromatographic process between HPLC and UPLC |
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| ZHOU Xin,CHEN Hui-ming,BAI Hua,YU Wen-lian,WANG Chao,ZHENG Jian,ZHANG Qing,CHEN Wei,MA Qiang.Study on conversion of chromatographic process between HPLC and UPLC[J].Chinese Journal of Analysis Laboratory,2008,27(4):56-58. |
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| Authors: | ZHOU Xin CHEN Hui-ming BAI Hua YU Wen-lian WANG Chao ZHENG Jian ZHANG Qing CHEN Wei MA Qiang |
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| Abstract: | The conversion of chromatographic process between HPLC and UPLC was studied on the basis of separation and determination of hydroxybenzoate preservatives in cosmetics.The optimal chromatographic conditions of UPLC was selected by comparison of chromatographic conditions of different methods.4 hydroxybenzoate preservatives in four kinds of cosmetics were determined quantitatively by HPLC and UPLC,and the results coincided with each other,indicating that two methods are accurate. |
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| Keywords: | Ultra performance liquid chromatography(UPLC) Conversion of chromatographic process Cosmetic Preservative |
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