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| 固定Fe 3＋的磁性微球分离富集鼠肝中铁结合蛋白的初步研究 | |
| 引用本文： | 方彩云a 王 胜a 刘清萍a 晏国全a 陆豪杰,a,b,c 杨芃原,a,b,c . 固定Fe 3＋的磁性微球分离富集鼠肝中铁结合蛋白的初步研究[J]. 化学学报, 2008, 66(19): 2174-2177 |
| 作者姓名： | 方彩云a 王 胜a 刘清萍a 晏国全a 陆豪杰 a b c 杨芃原 a b c |
| 作者单位： | (a复旦大学化学系 上海 200433) ;(b复旦大学生物医学研究院 上海 200032) ;(c复旦大学教育部创新科学仪器工程中心 上海 200433) ; |
| 摘 要： | 采用键合Fe3＋的纳米材料分离富集了大鼠肝脏中的铁结合蛋白质组, 并进行了质谱分析. 在相同的起始富集蛋白质量以及相同的吸附和洗脱条件下, 键合了Fe3＋的磁性纳米材料比未键合金属离子的空白材料富集了更多的蛋白质, 经质谱鉴定得到42个蛋白质, 主要包括代谢酶类、呼吸链主要成员、氧化还原蛋白、转运蛋白、血红蛋白等. |
| 关 键 词： | 磁性纳米材料 铁结合 蛋白质组 |
| 收稿时间： | 2008-02-04 |
| 修稿时间： | 2008-05-12 |
Preliminary Study of Iron-binding Proteins in Rat Liver by Magnetic Microsphere Immobilized Iron Affinity Chromatography |
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| Affiliation: | (a Department of Chemistry, Fudan University, Shanghai 200433) (b Institute of Biomedical Sciences, Fudan University, Shanghai 200032) (c Education Ministry Engineering Center for Innovative Scientific-instrument, Fudan University, Shanghai 200433) |
| Abstract: | Iron-binding proteome of rat liver were enriched by Fe3＋-immobilized silica nanomaterials and analyzed by HPLC-MS. More proteins were identified with enrichment of the Fe3＋-immobilized silica nanomaterials than those with normal silica nanomaterials when the starting proteins with the same amount and the same conditions of absorption and elution were used. Finally, 42 iron-binding proteins were identified, which were mainly referred to enzymes of metabolism, members of respiratory chain, proteins involved in oxidation-reduction, transporters and hemoglobins. |
| Keywords: | magnetic nanomaterial iron-binding proteome |
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