Cascade Reactions in Multicompartmentalized Polymersomes |
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| Authors: | Ruud J. R. W. Peters Maïté Marguet Sébastien Marais Prof. Marco W. Fraaije Prof. Jan C. M. van Hest Prof. Sébastien Lecommandoux |
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| Affiliation: | 1. Radboud University Nijmegen, Institute for Molecules and Materials, Bio‐organic Chemistry, Heyendaalseweg 135, 6525 AJ, Nijmegen (The Netherlands);2. Université de Bordeaux, LCPO, UMR 5629, 33600 Pessac (France);3. CNRS, LCPO, UMR 5629, 33600 Pessac (France);4. Bordeaux Imaging Center Université Bordeaux Segalen, CNRS UMS3420—Inserm US004, Institut Fran?ois Magendie, 146 rue Léo‐Saignat, 33077 Bordeaux Cedex (France);5. University of Groningen, Biomolecular Sciences and Biotechnology Institute, Laboratory of Biochemistry, Nijenborgh 4, 9747 AG Groningen (The Netherlands) |
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| Abstract: | Enzyme‐filled polystyrene‐b‐poly(3‐(isocyano‐L ‐alanyl‐aminoethyl)thiophene) (PS‐b‐PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene‐b‐poly(ethylene oxide) (PB‐b‐PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor‐dependent three‐enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments. |
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| Keywords: | artificial cells cascade reactions enzyme catalysis nanoreactors polymersomes |
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