Three‐Dimensional Protein Networks Assembled by Two‐Photon Activation |
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| Authors: | Dr. Volker Gatterdam Dr. Radhan Ramadass Dr. Tatjana Stoess Manuela A. H. Fichte Prof. Dr. Josef Wachtveitl Prof. Dr. Alexander Heckel Prof. Dr. Robert Tampé |
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| Affiliation: | 1. Institute of Biochemistry, Biocenter, Goethe‐University Frankfurt, Max‐von‐Laue‐Strasse 9, 60438 Frankfurt am Main (Germany) http://www.biochem.uni‐frankfurt.de;2. Institute of Physical and Theoretical Chemistry, Goethe‐University Frankfurt (Germany);3. Institute of Organic Chemistry and Chemical Biology, Goethe‐University Frankfurt (Germany) |
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| Abstract: | Spatial and temporal control over chemical and biological processes plays a key role in life and material sciences. Here we synthesized a two‐photon‐activatable glutathione (GSH) to trigger the interaction with glutathione S‐transferase (GST) by light at superior spatiotemporal resolution. The compound shows fast and well‐confined photoconversion into the bioactive GSH, which is free to interact with GST‐tagged proteins. The GSH/GST interaction can be phototriggered, changing its affinity over several orders of magnitude into the nanomolar range. Multiplexed three‐dimensional (3D) protein networks are simultaneously generated in situ through two‐photon fs‐pulsed laser‐scanning excitation. The two‐photon activation facilitates the three‐dimensional assembly of protein structures in real time at hitherto unseen resolution in time and space, thus opening up new applications far beyond the presented examples. |
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| Keywords: | immobilization photochemistry protein– protein interactions surface chemistry two‐photon activation |
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