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Probing the Intracellular Glutathione Redox Potential by In‐Cell NMR Spectroscopy |
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| Authors: | Dr Steve Y Rhieu Dr Aaron A Urbas Dr Daniel W Bearden Dr John P Marino Dr Katrice A Lippa Dr Vytas Reipa |
| Institution: | 1. Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA);2. Hollings Marine Laboratory, Chemical Sciences Division, National Institute of Standards and Technology, Charleston, SC 29412 (USA);3. Institute for Bioscience and Biotechnology Research, Biomolecular Measurement Division, National Institute of Standards and Technology, Rockville, MD 20850 (USA);4. Chemical Sciences Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 (USA) |
| Abstract: | Non‐invasive and real‐time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in‐cell nuclear magnetic resonance (NMR) based method for measuring the intracellular glutathione redox potential by direct and quantitative measurement of isotopically labeled glutathione introduced exogenously into living yeast. By using this approach, perturbations in the cellular glutathione redox homeostasis were also monitored as yeast cells were subjected to oxidative stress. |
| Keywords: | glutathione living cells NMR spectroscopy oxidative stress redox chemistry |