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Inhibition of Heme Uptake in Pseudomonas aeruginosa by its Hemophore (HasAp) Bound to Synthetic Metal Complexes |
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| Authors: | Chikako Shirataki Dr. Osami Shoji Mitsuyoshi Terada Prof. Dr. Shin‐ichi Ozaki Dr. Hiroshi Sugimoto Prof. Dr. Yoshitsugu Shiro Prof. Dr. Yoshihito Watanabe |
| Affiliation: | 1. Department of Chemistry, Graduate School of Science, Nagoya University, Furo‐cho, Chikusa‐ku, Nagoya 464‐8602 (Japan);2. Department of Biological Science, Yamaguchi University, 1677‐1 Yoshida, Yamaguchi 753‐8515 (Japan);3. RIKEN SPring‐8 Center, Harima Institute, 1‐1‐1 Kouto, Sayo, Hyogo 679‐5148 (Japan);4. Research Center for Materials Science, Nagoya University, Furo‐cho, Chikusa‐ku, Nagoya 464‐8602 (Japan) http://bioinorg.chem.nagoya‐u.ac.jp/ |
| Abstract: | The heme acquisition system A protein secreted by Pseudomonas aeruginosa (HasAp) can capture several synthetic metal complexes other than heme. The crystal structures of HasAp harboring synthetic metal complexes revealed only small perturbation of the overall HasAp structure. An inhibitory effect upon heme acquisition by HasAp bearing synthetic metal complexes was examined by monitoring the growth of Pseudomonas aeruginosa PAO1. HasAp bound to iron–phthalocyanine inhibits heme acquisition in the presence of heme‐bound HasAp as an iron source. |
| Keywords: | growth inhibition HasA heme proteins iron complexes protein structures |