|
|||||
|
|
Irreversible Site‐Specific Hydrazinolysis of Proteins by Use of Sortase |
|
| Authors: | Yi‐Ming Li Yi‐Tong Li Man Pan Xiu‐Qi Kong Yi‐Chao Huang Prof. Zhang‐Yong Hong Prof. Lei Liu |
| Affiliation: | 1. Tsinghua‐Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084 (China);2. School of Medical Engineering, Hefei University of Technology, Hefei, Anhui 230009 (China);3. The College of Life Sciences, Nankai University, Tianjing 300071 (China) |
| Abstract: | Sortase‐mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C‐terminal modification of proteins with functional units as well as for the preparation of C‐to‐C fusion proteins. |
| Keywords: | hydrazones hydrolysis ligation protein modification proteins |