Pinpointing Double Bonds in Lipids by Paternò‐Büchi Reactions and Mass Spectrometry |
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Authors: | Dr. Xiaoxiao Ma Prof. Dr. Yu Xia |
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Affiliation: | Department of Chemistry, Purdue University, 560 Oval Dr., West Lafayette, IN (USA) http://www.chem.purdue.edu/xia/ |
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Abstract: | The positions of double bonds in lipids play critical roles in their biochemical and biophysical properties. In this study, by coupling Paternò–Büchi (P‐B) reaction with tandem mass spectrometry, we developed a novel method that can achieve confident, fast, and sensitive determination of double bond locations within various types of lipids. The P‐B reaction is facilitated by UV irradiation of a nanoelectrospray plume entraining lipids and acetone. Tandem mass spectrometry of the on‐line reaction products via collision activation leads to the rupture of oxetane rings and the formation of diagnostic ions specific to the double bond location. |
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Keywords: | double bond location Paternò – Bü chi reaction photochemical reactions tandem mass spectrometry unsaturated lipids |
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