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Lactose as a “Trojan Horse” for Quantum Dot Cell Transport |
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| Authors: | Dr David Benito‐Alifonso Shirley Tremel Bo Hou Harriet Lockyear Dr Judith Mantell Prof David J Fermin Dr Paul Verkade Dr Monica Berry Dr M Carmen Galan |
| Institution: | 1. School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS (UK);2. Wolfson Bioimaging Facility, School of Biochemistry and Physiology & Pharmacology, Medical Sciences Building, University of Bristol, University Walk, Bristol BS8 1TD (UK);3. School of Physics, University of Bristol, NSQI, Tyndall Ave, Bristol BS8 1F (UK) |
| Abstract: | A series of glycan‐coated quantum dots were prepared to probe the effect of glycan presentation in intracellular localization in HeLa and SV40 epithelial cells. We show that glycan density mostly impacts on cell toxicity, whereas glycan type affects the cell uptake and intracellular localization. Moreover, we show that lactose can act as a “Trojan horse” on bi‐functionalized QDs to help intracellular delivery of other non‐internalizable glycan moieties and largely avoid the endosomal/lysosomal degradative pathway. |
| Keywords: | glycans intracellular localization multivalency nanoparticles quantum dots |