| 低聚核苷酸d(GGTATACC)2的1H和13C NMR研究 |
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| 引用本文： | 阎江丽 张友杰. 低聚核苷酸d(GGTATACC)2的1H和13C NMR研究[J]. 波谱学杂志, 1998, 15(2): 117-122 |
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| 作者姓名： | 阎江丽 张友杰 |
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| 作者单位： | 1.中国科学院武汉物理与数学研究所波谱与原子分子物理国家重点实验室, 武汉 430071;2.华中师范大学分析测试中心, 武汉 430079 |
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| 摘 要： | 通过综合分析2D 1H NOE谱(混合时间100ms),13C-1H异核多量子化学位移相关谱和1H双量子滤波化学位移相关谱归属了低聚脱氧核糖核酸片断d(GGTATACC)2 1H和13C的化学位移.该8聚核苷酸在溶液中为双螺旋B型结构,各碱基相对于糖环呈反式构象,糖环本身则均以C2'-endo构象为主.
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| 关 键 词： | d(GGTATACC)2 1H化学位移 13C 化学位移 NOESY HMQC |
| 收稿时间： | 1997-12-24 |
1H AND 13 C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC) 2 IN SOLUTION |
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| Yan Jiangli a,b,Zhang Youjie b,Yuan Hanzhen a,Mao Xi an a and Shen Lianfang a. 1H AND 13 C NMR STUDY OF DEOXYOLIGONUCLEOTIDE d(GGTATACC) 2 IN SOLUTION[J]. Chinese Journal of Magnetic Resonance, 1998, 15(2): 117-122 |
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| Authors: | Yan Jiangli a b Zhang Youjie b Yuan Hanzhen a Mao Xi an a Shen Lianfang a |
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| Affiliation: | 1.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, The Chinese Academy of Sciences, Wuhan 430071;2.Center for Analysis and Testing, Central China Normal University, Wuhan 430079 |
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| Abstract: | Deoxyoligo nucleotide d(GGTATACC)2 was studied by two dimensional NMR techniques. Its proton and 13C chemical shifts were assigned by 2D proton NOE spectrum and 13C-1H heteronuclear multiquantum chemical shift correlated spectrum (HMQC) combined with double quantum filtered chemical shift correlated spectrum (DQFCOSY). The sugar puckers of the eight residues of this helix octamer were determined to be dominantly C2'-endo forms with anti glycosidic conformations. |
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| Keywords: | d(GGTATACC) 2 1H and 13 C chemical shifts NOESY HMQC |
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