Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region |
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Authors: | Egawa Takahiro Koide Yuichiro Hanaoka Kenjiro Komatsu Toru Terai Takuya Nagano Tetsuo |
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Institution: | Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
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Abstract: | We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells. |
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