Development of a system to sensitively and specifically visualize c-fos mRNA in living cells using bispyrene-modified RNA probes |
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| Authors: | Waki Reiko Yamayoshi Asako Kobori Akio Murakami Akira |
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| Affiliation: | Department of Biomolecular Engineering, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. |
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| Abstract: | In situ visualization of c-fos mRNA was shown both in fixed cells and in living cells using bispyrene-modified 2'-O-methyl RNA probes (OMUpy2) or phosphorothioate modified OMUpy2 (OMUpy2-S), which was RNA-specific with high sensitivity, and a system for time-lapse imaging of c-fos mRNA was successfully developed. |
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