Peptide immobilization on amine-terminated boron-doped diamond surfaces |
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Authors: | Coffinier Yannick Szunerits Sabine Jama Charafeddine Desmet Rémi Melnyk Oleg Marcus Bernadette Gengembre Léon Payen Edmond Delabouglise Didier Boukherroub Rabah |
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Institution: | Institut de Recherche Interdisciplinaire (IRI, FRE2963) and Institut d'Electronique, de Microélectronique et de Nanotechnologie (IEMN), Cité Scientifique, Avenue Poincaré - BP. 60069, 59652 Villeneuve d'Ascq, France. |
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Abstract: | This paper reports on the formation and characterization of semicarbazide termination on aminated boron-doped diamond (BDD) surfaces, and further preparation of peptide microarray through site-specific alpha-oxo semicarbazone ligation. Hydrogen-terminated BDD electrodes were first aminated using NH3 plasma treatment and then reacted with triphosgene and Fmoc-protected hydrazine to yield a protected semicarbazide termination. Subsequent deprotection and chemical reaction with glyoxylyl peptides led to the covalent immobilization of the peptides on the surface through site-specific ligation. The resulting surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and fluorescence measurements. |
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