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Terahertz absorption spectroscopy of protein-containing reverse micellar solution
Authors:H. Murakami  Y. Toyota  T. Nishi  S. Nashima
Affiliation:1. Kansai Photon Science Institute, Japan Atomic Energy Agency, Kyoto 619-0215, Japan;2. Research Unit for Quantum Beam Life Science Initiative, Japan Atomic Energy Agency, Kyoto 619-0215, Japan;3. Department of Applied Physics, Osaka City University, Osaka 558-8585, Japan;4. Department of Chemistry and Materials Technology, Kyoto Institute of Technology, Kyoto 606-8585, Japan
Abstract:Terahertz time-domain spectroscopy has been carried out for AOT/isooctane reverse micellar solution with myoglobin at the water-to-surfactant molar ratios (w0) of 0.2 and 4.4. The amplitude of the absorption spectrum increases with increasing the protein concentration at w0 = 0.2, whereas it decreases at w0 = 4.4. The molar extinction coefficients of the protein-filled reverse micelle, and the constituents, i.e., myoglobin, water, and AOT, have been derived by use of the structural parameters of the micellar solution. The experimental results are interpreted in terms of hydration onto the protein and surfactant in the reverse micelle.
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