|
|||||
|
|
Comparison of separation modes of high-performance liquid chromatography for the analysis of glycoprotein- and proteoglycan-derived oligosaccharides |
|
| Authors: | Michael J Davies Elizabeth F Hounsell |
| Institution: | Glycoprotein Structure/Function Group, Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, UK |
| Abstract: | Porous graphitised carbon (PGC) has been explored for high-performance liquid chromatography (HPLC) of mono- and di-saccharides released from proteoglycans and of fluorescently labelled oligosaccharide derivatives for high-sensitivity detection. Sulphated oligosaccharides show good retention and separation behaviour on PGC-HPLC, and compared to anion-exchange or reversed-phase ion-pair chromatography the chromatography is carried out in the absence of salt. Due to their poor retention on PGC-HPLC the analysis of single uronic acids has been optimised with high pH anion-exchange chromatography. Fluorescent labelled derivatives formed by reductive amination of neutral oligosaccharides with 2-aminobenzamide have been chromatographed on PGC-HPLC and by BioGel P4 gel filtration. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |