A HPLC method for specific determination ofα-amylase and glucoamylase in complex enzymatic preparations |
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Authors: | J M Franco M A Murado Maria I G Siso J Miron Maria P Gonzalez |
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Institution: | (1) Instituto de Investigaciones Marinas (CSIC), c/Eduardo Cabello, 6, 36208 Vigo, Spain |
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Abstract: | Summary In the hydrolysis of soluble starch by mixtures of α-amylase and glucoamylase, the ratios maltose/glucose and maltoriose/glucose
linearly depend, over a wide range, on the relation between both enzymes and are independent on the activity level of the
enzymatic preparation. HPLC determination of hydrolysis products (glucose, maltose and maltotriose) of soluble starch by mixtures
of these enzymes, after incubation under controlled conditions, is a rapid method for the evaluation of the relative levels
of each enzyme in the mixtures. The method, first developed using pure commercial amylases, is applied, with consistent results,
to cell free media ofAspergillus niger cultures on a glycogen-rich effluent. |
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Keywords: | Column liquid chromatography Glucose maltose and maltotriose α -Amylase and glucoamylase Microfungal cultures |
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