Comparison of separation performances of amylose- and cellulose-based stationary phases in the high-performance liquid chromatographic enantioseparation of stereoisomers of β-lactams |
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| Authors: | Zoltán Pataj István Ilisz Róbert Berkecz Enik? Forró Ferenc Fülöp Antal Péter |
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| Institution: | 1. Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, H-6720 Szeged, Hungary;2. Institute of Pharmaceutical Chemistry, University of Szeged, Eötvös utca 6,H-6720 Szeged, Hungary |
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| Abstract: | High-performance liquid chromatographic methods were developed for the separation of the enantiomers of 19 β-lactams. The direct separations were performed on chiral stationary phases containing either amylose tris(3,5-dimethylphenylcarbamate), (Kromasil® AmyCoat? column) or cellulose tris(3,5-dimethylphenylcarbamate), (Kromasil® CelluCoat? column) as chiral selector. The different methods were compared in systematic chromatographic examinations. The separations were carried out with good selectivity and resolution. The AmyCoat? and CelluCoat? columns appear to be highly complementary. The best separations of bi- and tricyclic β-lactam stereoisomers were obtained with the AmyCoat? column, while the 4-aryl-substituted β-lactams were better separated on the CelluCoat? column. The elution sequence was determined in all cases; no general rule could be established. |
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