PHOTOBLEACHING DIFFERENCE ABSORPTION SPECTRA OF HUMAN CONE PIGMENTS: QUANTITATIVE ANALYSIS AND COMPARISON TO OTHER METHODS |
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| Authors: | Shannath L. Merbs Jeremy Nathans |
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| Affiliation: | Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205. |
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| Abstract: | Four human cone pigment apoproteins were expressed by transfection of human tissue culture cells with the corresponding complementary DNA clones. Following reconstitution of the cone pigments by incubation with 11-cis retinal, photobleaching difference absorption spectra were obtained for the blue pigment, the green pigment, and two polymorphic variants of the red pigment. These spectra were analyzed to determine the wavelengths of maximal absorbance and the bandwidths. The recombinant cone pigment spectra were compared to human cone spectral sensitivities and cone pigment absorption spectra determined by microspectrophotometry, single-cell electrophysiology, reflection densitometry, electroretinography, and psychophysical color and brightness matching. |
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