肿瘤靶向PEI包覆磁性纳米凝胶的光化学制备及表征

以聚乙烯亚胺(Polyethyleneimine, PEI)为预聚体, H2O2为抽氢剂, 采用光化学方法制备了PEI包覆的磁性纳米凝胶(PEI-Fe3O4), 在PEI-Fe3O4表面共价偶联具有肿瘤靶向作用的叶酸分子(Folate, FA), 制备得到肿瘤靶向PEI包覆磁性纳米凝胶(FA-PEI- Fe3O4). 采用多种手段对PEI-Fe3O4及FA-PEI-Fe3O4进行了表征. 结果表明, FA-PEI-Fe3O4平均粒径为140 nm, 形状规则, 具有超顺磁性, 磁含量达到22.4%, 饱和磁化强度达65.6 A·m2·kg-1, 磁性纳米凝胶较高的磁响应性能和具有肿瘤靶向作用的叶酸分子的修饰, 为其作为潜在的肿瘤靶向载体奠定了基础.

Photochemical Synthesis and Characterization of Polyethyleneimine-coated Magnetic Nanogel Used as Cancer-targeted Carrier

The polyethyleneimine(PEI)-coated magnetic nanogel was synthesized via photochemical polymerization under UV-light irradiation. Modified with folic acid, which was expected to be able to identify tumor surface receptors and to have a high binding force with cancer cells, the FA-PEI-Fe3O4 nanogel was prepared. The FA-PEI-FeeO4 nanogel was characterized by Fourier transform infrared spectroscopy, thermogravimetric analysis, scan electron microscope and vibrating sample magnetometer, respectively. The results showed that the magnetic nanogel was regular shape with a mean diameter of 140 nm. With high saturation magnetization and folate modification, the FA-PEI-Fe3O4 nanogel suggests promising potential application in cancer-targeted drug delivery.