Development of the Fingerprints for the Quality Evaluation of Scutellariae Radix by HPLC-DAD and LC-MS-MS |
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Authors: | Lei Zhang Ruo-Wen Zhang Qing Li Jian-Wei Lian Jian Liang Xiao-Hui Chen Kai-Shun Bi |
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Affiliation: | 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, People’s Republic of China 2. College of Chinese Traditional Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, 510006, Guangdong, People’s Republic of China 3. Sichuan University College of Preclinical Medicine and Forensic Medicine, Chengdu, 610041, People’s Republic of China
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Abstract: | A high-performance liquid chromatography coupled with photodiode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS2) method was firstly developed for a chemical fingerprint analysis of Scutellariae Radix and rapid identification of major compounds in the fingerprints. The experimental data for chromatography were used for hierarchical clustering analysis and similarity calculation, and those for UV and MS spectra were applied for identifying characteristic peaks. Twenty samples including S. baicalensis Georgi., S. viscidula Bge. and S. amoena C. H. Wright were classified into three groups. By comparing the UV and MS spectra data with those of the authentic standards and literature, 20 main peaks in the fingerprints were identified for the first time. The developed fingerprint assay was specific and could be readily utilized for comprehensive evaluation of Scutellariae Radix. |
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