Thermal Gradients for the Control of Elution in RP-LC: Application to the Separation of Model Drugs |
| |
Authors: | Anthony M. Edge Ian D. Wilson Stefanie Shillingford |
| |
Affiliation: | 1. Department of Drug Metabolism and Pharmacokinetics, AstraZeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire, SK10 4TG, UK
|
| |
Abstract: | The use of temperature as a variable in liquid chromatography enables the facile alteration of eluotropic strength without the need to change solvent composition. The ability to change eluotropic strength via temperature alone means that thermal gradients can be used to mimic the effects of solvent gradients but without many of the unwanted effects of changes in solvent composition. Here we illustrate the use of thermal gradients as a means of controlling chromatographic separations using either constant flow or, with the flow rate increased to maintain isobaric conditions, constant pressure, performed using columns packed with 1.7 μm particles. A model is described that can be used to used to predict flow, pressure and temperature under gradient conditions. Practical experimental factors such as the need for post column cooling and the use of frit restrictors in order to obtain optimum results are described. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|