Simultaneous HPLC Determination of Four Key Metabolites in the Metabolic Pathway for Production of 1,3-Propanediol from Glycerol |
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Authors: | Hongwen Chen Baishan Fang Zongding Hu |
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Affiliation: | 1. Key Laboratory of Industrial Biotechnology, Huaqiao University, Fujian Province University, Quanzhou, Fujian, 362021, China 2. College of Chemical Engineering, Tianjin University, 300072, Tianjin, China
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Abstract: | A high-performance liquid chromatographic (HPLC) method with refractive-index detection has been developed for simultaneous analysis of glycerol, dihydroxyacetone (DHA), 3-hydroxypropionaldehyde (3-HPA), and 1,3-propanediol (1,3-PD), four key substances in the metabolic pathway for production of 1,3-PD from glycerol by microorganism fermentation. The compounds were separated on a 300 mm × 7.8 mm ion-exclusion column with a 65:35 (v/v) mixture of deionized water and acetonitrile, containing 0.0005 M H2SO4, as mobile phase. The flow rate was 0.5 mL min?1. Under these conditions the retention times of 3-HPA, DHA, glycerol, and 1,3-PD were 6.87, 14.63, 16.37, and 18.50 min, respectively. Relative standard deviations and average recoveries were between 0.42 and 0.63% and between 96.7 and 103.1%, respectively; detection limits were between 0.017 and 0.038 g L?1. The method enabled separation of these compounds. |
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