HPLC–MS Analysis of Isoniazid in Dog Plasma |
| |
Authors: | Anna Wang Wei Zhang Jin Sun Jianfang Liu Yanshuang Sang Shuo Gao Zhonggui He |
| |
Affiliation: | 1. Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China
|
| |
Abstract: | A simple, rapid, and sensitive liquid chromatography–mass spectrometric (LC–MS) method was developed and validated for the determination of isoniazid in dog plasma. Plasma samples were deproteined with methanol and separated on a C18 column interfaced with a single quadrupole mass spectrometer, using 0.1% formic acid–acetonitrile (91:9 v/v) as mobile phase. Detection was performed by positive electrospray ionization with selected ion monitoring at m/z 138 for isoniazid and 152 for entecavir maleate internal standard. Linearity was obtained over the range of 25–5,000 ng mL?1, with a lower limit of quantification of 25 ng mL?1. The intra- and inter-day precision was less than 2.7% in terms of relative standard deviation. Accuracy, expressed as relative error, ranged from ?2.0 to 8.0%. Plasma samples were analysed within 5 min. The method was successfully applied to the evaluation of the pharmacokinetics of isoniazid in dog plasma. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|