HPTLC Method for Determination of 20-Hydroxyecdysone in Sida rhombifolia L. and Dietary Supplements |
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| Authors: | Atul N. Jadhav Chidananda Swamy Rumalla Bharathi Avula Ikhlas A. Khan |
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| Affiliation: | 1. National Center for Natural Products Research, University of Mississippi, University, MS, 38677, USA
2. Department of Pharmacognosy, School of Pharmacy, University of Mississippi, University, MS, 38677, USA
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| Abstract: | Naturally occurring 20-hydroxyecdysone is an important anabolic ecdysteroid. A simple thin-layer chromatography method to quantitate 20-hydroxyecdysone in methanolic extract of the whole plant material of Sida rhombifolia L. was developed. This method was successfully applied for quantitative evaluation of dietary supplements. The separation was achieved on glass TLC plates coated with silica gel 60F254, using chloroform: methanol (8:2 v/v) as developing solvent. Densitometric evaluation of 20-hydroxyecdysone was performed at 250 nm in reflectance/absorbance mode. The calibration was in the range of 200–1,000 ng spot?1 and correlation coefficient for the calibration curve was >0.999. In addition, for six different Sida species unique fingerprints were obtained on the HPTLC plate. |
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