Metabolomic fingerprint classification of Brachychiton acerifolius organs via UPLC-qTOF-PDA-MS analysis and chemometrics |
| |
Authors: | Mohamed A Farag Aisha H Abou Zeid Manal A Hamed Zeinab Kandeel Hanaa M El-Rafie Radwa H El-Akad |
| |
Institution: | 1. Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini Street, P.B. 11562, Cairo, Egypt;2. Pharmacognosy Department, National Research Center, Dokki, Cairo 12311, Egypt;3. Therapeutic Chemistry Department, National Research Center, Dokki, Cairo 12311, Egypt |
| |
Abstract: | Brachychiton acerifolius, or Sterculia acerifolia as formerly known, is a member of a genus reported for a myriad of bioactive compounds. Metabolome analysis of B. acerifolius – leaves, flowers and seeds – and quantification of its major compounds are demonstrated in this study. Metabolites were analysed via UPLC-PDA-qTOF-( ± ) ESI-MS and UPLC/ITMS, with a total of 56 metabolites characterised including 30 flavonoids, 2 anthocyanins, 6 phenolic acids (i.e. citric and hydroxycitric acid conjugates) and 8 fatty acids (FAs). Multivariate data analyses (i.e. principle component analysis and orthogonal partial least square-discriminate analysis) were applied to identify metabolite markers for each organ. Pelargonidin-O-glucoside and naringenin-O-glucuronide were found exclusively in flowers versus flavone enrichment in leaves (i.e. luteolin-O-glucuronide and apigenin-O-rhamnosyl glucuronide). Gas chromatography/mass spectrometry analysis revealed the presence of toxic cyclopropene FAs in seeds which may restrict its use. Antioxidant activity assessment for the three organs was performed in comparison with vitamin C as positive control. Leaves showed the highest activity (IC50 0.015 mg/mL). |
| |
Keywords: | Brachychiton acerifolius UPLC/MS analysis chemometrics antioxidant activity |
|
|