Preparative isolation of paclitaxel and related taxanes from cell cultures of Taxus chinensis using reversed-phase flash chromatography |
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Authors: | Zhi-Kun Liang Ruo-Gu Huang Zhi-Sheng Xie |
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Affiliation: | 1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, P.R. China;2. Guangdong Technology Research Centre for Advanced Chinese Medicine, Guangzhou 510006, P.R. China |
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Abstract: | In this study, paclitaxel, baccatin III, taxuyunnanine C and sinenxane C were successfully separated by reversed-phase flash chromatography on a manually packed C18 column from Taxus chinensis cell culture extract. The crude cell culture extract was first treated with Al2O3 column chromatography and then divided into two parts: fraction 1 and fraction 2. Ten milligrams of baccatin III and 19 mg of paclitaxel were obtained from 100 mg dried fraction 1. Fifty-two milligrams of taxuyunnanine C and 11 mg sinenxane C were obtained from 100 mg dried fraction 2. The purities of the four compounds were 98.02%, 98.53%, 98.93% and 98.76%, respectively. Their structures were characterised by using UV, MS and NMR. These results indicate that paclitaxel and related taxanes including baccatin III can be obtained from cell culture in a highly pure state using reversed-phase flash chromatography. |
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Keywords: | Taxus chinensis cell culture baccatin III paclitaxel taxuyunnanine C sinenxane C reversed-phase flash chromatography |
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