Cytotoxic constituents of Abutilon indicum leaves against U87MG human glioblastoma cells |
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| Authors: | Rukaiyya Sirajuddin Khan Mahibalan Senthi Poorna Chandra Rao Ameer Basha Mallika Alvala Dinesh Tummuri |
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| Institution: | 1. Department of Pharmacy, BITS-Pilani Hyderabad Campus, Jawahar Nagar, Shamirpet Mandal, Hyderabad 500 078, Telangana, India;2. Regional Agricultural Research Station (ANGRAU), Palem, Mahabub Nagar, Telangana, India;3. National Institute of Pharmaceutical Education and Research-Hyderabad, Balanagar, Hyderabad 500 037, Telanagana, India |
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| Abstract: | The study was aimed to identify cytotoxic leads from Abutilon indicum leaves for treating glioblastoma. The petroleum ether extract, methanol extract (AIM), chloroform and ethyl acetate sub-fractions (AIM-C and AIM-E, respectively) prepared from AIM were tested for cytotoxicity on U87MG human glioblastoma cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. These extracts exhibited considerable activity (IC50 values of 42.6–64.5 μg/mL). The most active AIM-C fraction was repeatedly chromatographed to yield four known compounds, methyl trans-p-coumarate (1), methyl caffeate (2), syringic acid (3) and pinellic acid (4). Cell viability assay of 1–4 against U87MG cells indicated 2 as most active (IC50 value of 8.2 μg/mL), whereas the other three compounds were much less active. Interestingly, compounds 1–4 were non-toxic towards normal human cells (HEK-293). The content of 2 in AIM-C was estimated as 3% by HPLC. Hence, presence of some more active substances besides methyl caffeate (2) in AIM-C is anticipated. |
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| Keywords: | Abutilon indicum MTT U87MG methyl caffeate cytotoxicity Malvaceae |
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