Placental extracts induce the expression of antioxidant enzyme genes and suppress melanogenesis in B16 melanoma cells |
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Authors: | Masahiro Yamasaki Shinya Hasegawa Hiroshi Takahashi Yuka Kobayashi Chihiro Sakai Yoko Ashizawa |
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Affiliation: | 1. Department of Health Chemistry, Hoshi University, 2-4-41, Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan;2. Snowden Corporation, Chiyoda-Ku, Tokyo 101-0031, Japan |
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Abstract: | One of the activities of placental extracts (PEs) is skin-whitening effect, but the physiological and genetic mechanism for this effect has not yet been clarified. Here, we focus on PE as a regulator of antioxidant enzyme genes. Porcine PE was prepared, and its activity was investigated in B16 melanoma cells. PE treatment decreased the melanin content of UV-irradiated B16 cells in a dose-dependent manner. PE directly reduced the enzyme activity of tyrosinase in a cell-free assay. In addition, PE treatment increased the gene expression of cytosolic superoxide dismutase (SOD-1), extracellular SOD (SOD-3) and catalase but did not affect the expression of tyrosinase. Moreover, PE protected the B16 cells from H2O2-induced cell death. Taken together, our data suggest that PEs could play a role not only as a suppressor of melanin synthesis but also as a regulator of antioxidant genes and might protect the skin against oxidative stress. |
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Keywords: | placenta extracts melanin synthesis superoxide dismutase antioxidant enzyme |
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